Corning? basic Fibroblast Growth Factor (bFGF), human recombinant, 100μg, is a heparin-binding mitogenic protein that enhances the proliferation of a wide variety of cell types under serum-free or serum-reduced conditions. 10μg/container, 10/pack, formerly Cat. #40060B. These growth factors have been used to promote long-term proliferation in cell culture and to induce cell differentiation in a variety of cell types under serum-free or reduced serum conditions.
Lyophilized from phosphate buffered saline, carrier-free; reconstitute in dH2O or serum-free medium
Preparation and Storage
Stable for at least three months at -20°C (lyophilized) or = one month solubilized at -20°C. Do not store in frost-free freezer. Avoid multiple freeze/thaws.
Purity: >95% by SDS-PAGE
Tested for ability to promote proliferation of FBHEC
Filtered (0.2 μm membrane) and tested and found negative for bacteria, fungi and mycoplasma
Recombinant, E. coli
Recommended concentration: 1 to 20 ng/ mL of medium depending on cell type
Shelf life: >= 3 months from date of shipment at -20°C (lyophilized) or >= one month at -20°C (solubilized)